Skip to main content
. 2023 Dec 14;43(6):406–419. doi: 10.1038/s41388-023-02914-0

Fig. 2. PIM1 induces LD accumulation through GSK3β inhibition.

Fig. 2

A Representative images of LDs in PC3TripzPIM1 cells following doxycycline treatment (50 ng/ml, 24 h) alone, and in combination with PIM inhibition (PIM447, 3 µM, 24 h). B Western blotting of PC3Tripz-PIM1 cells following doxycycline treatment (50 ng/ml, 24 h) alone, and in combination with PIM inhibition (PIM447, 3 µM, 24 h). C Quantification of LD size and number per nuclei (n > 30 cells/group). D Representative images of LDs and (E) western blotting of PC3TripzPIM1 cells following doxycycline treatment (50 ng/ml, 24 h) expressing vector or GSK3β (S9A) mutant. F Quantification of LD size and number per nuclei (n > 30 cells/group). For staining in (A) and (D), LDs in green (LipidSpot488) and nuclei in blue (Dapi). Scale bars, 50 µm. n = 3, mean ± SEM. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.