Skip to main content
. 2023 Dec 14;43(6):406–419. doi: 10.1038/s41388-023-02914-0

Fig. 6. PIM1 promotes cell survival during nutrient stress.

Fig. 6

A Quantitative analysis of SyTox fluorescence intensity indicating relative cell death in the indiated cell lines maintained in complete media (CM, RPMI1640, 10% dialyzed FBS, 25 mM Glucose) or depleted media (DM, RPMI1640, 10% dialyzed FBS) for 24 h. B Representative images of LDs in PC3LN4pCIP and PC3LN4hPIM1 cells maintained in complete or depleted media for 48 h. LDs in green (LipidSpot488) and nuclei in blue (Dapi). Scale bars, 50 µm. C Quantification of LD size and number per nuclei from (n > 30/treatment group). D Quantitative analysis of SyTox fluorescence intensity indicating relative cell death in the indicated cell lines maintained in complete or depleted media and treated with DMSO or Etomoxir (ETO, 100 µM, 48 h). E Representative images of crystal violet staining of PC3LN4pCIP, PC3LN4hPIM1, DU145pCIP, and DU145hPIM1 cells maintained in complete or depleted media and treated with DMSO or ETO. F Quantification of crystal violet staining. n = 3, mean ± SEM. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.