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. 2024 Feb 2;15:988. doi: 10.1038/s41467-024-45283-2

Fig. 6. Intercellular mitochondrial transfer via TNTs rescues import function.

Fig. 6

MitoLuc import trace (a) and amplitudes (b) following treatment with vehicle (DMSO; grey), chronic (48 h; orange), or acute (10 min; red) MB20. N = 3 biological replicates, each with 3 technical replicates. P = 0.3985, 0.0015, 0.0005. c Quantification of the proportion of cells with TNTs following chronic treatment with 10 µM MB20 (48 h). N = 6 biological replicates; 20 cells counted per replicate. P = 0.0015, 0.5479, 0.2957, 0. 0005. MitoLuc import trace (d) and amplitudes (e) in HeLaGAL cells subjected to no or chronic trapping (48 h) with vehicle (DMSO) or 100 nM nocodozole (48 h). N = 3 biological replicates. P = > 0.9999, 0.0276, 0.0448, 0.0283, 0.9845, 0.9867. Statistical significance was determined using one-way (b, e) or two-way (c) ANOVAs with Tukey’s tests. Data are presented as mean values ± S.D. with individual data points for each biological replicate, and P values are reported left-right bottom-top (b, c, e), or representative traces are shown (a, d). Raw data are provided in the Source Data file (a–e). MB20 MitoBloCK−20, MTS mitochondrial targeting sequence, MTX methotrexate, Noco nocodazole, TNT tunnelling nanotube.