Table 1.
Basic SAXS parameters Rg (radius of gyration), Dmax. (maximum size) and Rg(GNOM) (radius of gyration calculated using GNOM) derived from the SAXS profiles of the titration series of OaPAC with ATP (Fig. 3).
| [ATPfinal] | Molar ratio (ATP:OaPAC) | Rg(Å) (Guinier) /r2 (fit) | ΔRg(Å) (Guinier) | Rg(Å) (GNOM*) | ΔRg(Å) (GNOM) | Dmax (Å) /χ2 |
|---|---|---|---|---|---|---|
| 0 μΜ | – | 30.63 ± 0.31 /0.9790 | – | 30.65 ± 0.02 | – | 90 /1.0281 |
| 10 μΜ | 0.14 | 31.54 ± 0.48 /0.9873 | 0.91 | 30.44 ± 0.02 | −0.21 | 92 /1.0556 |
| 50 μΜ | 0.71 | 31.84 ± 0.67 /0.9857 | 1.21 | 31.04 ± 0.04 | 0.39 | 105 /1.2875 |
| 70 μΜ | 1.0 | 33.57 ± 0.41 /0.9921 | 2.94 | 33.21 ± 0.05 | 2.56 | 120 /1.2590 |
| 100 μΜ | 1.4 | 33.95± 0.62 /0.9902 | 3.32 | 32.81 ± 0.05 | 2.16 | 126 /1.0839 |
| 150 μΜ | 2.1 | 34.12 ± 0.39 /0.9957 | 3.49 | 33.94 ± 0.05 | 3.29 | 128 /1.0093 |
| 200 μΜ | 2.9 | 34.90 ± 0.38 /0.9937 | 4.27 | 34.31 ± 0.06 | 3.66 | 130 /1.0808 |
| 250 μΜ | 3.6 | 34.70 ± 0.61 /0.9903 | 4.07 | 34.28 ± 0.05 | 3.63 | 131 /1.0616 |
| 300 μΜ | 4.3 | 34.93 ± 0.38 /0.9906 | 4.3 | 34.02 ± 0.06 | 3.37 | 131 /1.1655 |
| 400 μΜ | 5.7 | 34.82 ± 0.50 /0.9930 | 4.19 | 34.43 ± 0.05 | 3.78 | 130 /1.0904 |
| 500 μΜ | 7.1 | 35.01 ± 0.66 /0.9888 | 4.38 | 34.16 ± 0.05 | 3.51 | 130 /1.1407 |
| 700 μΜ | 10.0 | 35.25 ± 0.65 /0.9867 | 4.62 | 33.93 ± 0.06 | 3.28 | 132 /1.1421 |
| 800 μΜ | 11.4 | 35.23 ± 0.45 /0.9913 | 4.6 | 33.67 ± 0.05 | 3.02 | 131 /1.1337 |
| 1000 μΜ | 14.3 | 35.91 ± 0.41 /0.9817 | 5.28 | 33.35 ± 0.05 | 2.7 | 131 /1.4938 |
GNOM22 is an indirect transform program for small-angle scattering data processing. It reads in one-dimensional scattering curves (possibly smeared with instrumental distortions) and evaluates the particle distance distribution function P(r). χ2 is the chi square. All residual plots are presented in Supplementary Fig. 4 (Supplementary Note 4).