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Thermal stability of OaPAC in the presence of ApCpp and ATP as measured by temperature dependent fluorescence (nanoDSF and emission of the flavin) and DSC.
| Nucleotides | Tm (oC) nanoDSF | Tm (oC) emission flavin | Tm (oC) DSC |
|---|---|---|---|
| no nucleotide | 66.31 ± 0.06 | 63.4 ± 0.5 | 67.2 |
| ApCpp | 69.5 ± 0.14 | 66.6 ± 0.19 | 71.1 |
| ATP | 68.46 ± 0.01 | n.m. | n.m |
| cAMP | 66.49 ± 0.01 | n.m. | n.m |
n.m. not measured
