Fig. 1. Transient expression of GFP fusions in wild-type tobacco protoplasts. (A) Expression of construct RpoT;2–GFP examined by CLSM. GFP fluorescence was detected in the green channel (left) and merged (right) with chlorophyll autofluorescence visible in the red channel. (B) Mitochondrial targeting control CoxIV–GFP (Akashi et al., 1998). (C) Plastid targeting control RecA–GFP (Akashi et al., 1998). The control images were taken by epifluorescence microscopy using GFP- (left) and FITC- (right) filter sets, respectively. Scale bars indicate 10 µm.