Fig. 2. Functional analysis of the two methionine residues in the N-terminus of RpoT;2. (A) Sequence comparison of of the three Arabidopisis RpoT gene products. Only the variable N-termini up to the motif RQVKXETE are shown. The alignment was performed using Multalin (Corpet, 1988); identical amino acid positions are highlighted. (B) Schematic presentation of the three different GFP fusions used in transient expression experiments (for details see Results). (C–E) Expression of RpoT;2–GFP (C), RpoT;2[M40I]–GFP (D), and RpoT;2[Δ39]–GFP (E) in protoplasts of the rpoB^– mutant of N. tabacum. Pictures were taken by CLSM in the green channel. Scale bars indicate 10 µm.