Fig. 3. Effects of wortmannin on the lysosomal degradation of EGFR in MDCK, SKBR-3 and BT20 cells. (A) Cells were treated with wortmannin and then stimulated with EGF for the indicated times. Cell lysates were subjected to immunoblot analysis with anti-EGFR or anti-phospho-Akt (p-Akt) antibodies. (B) Quantification of the results from (A). Quantification was performed with a digital imaging system from Alpha Innotech Corporation (San Leandro, CA).