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Fig. 4. Regulation of the intracellular trafficking of EGFR by Rab5. SKBR-3 cells were transfected with wild-type Rab5, mutant Rab5 S34N or Q79L for 48 h and then stimulated with EGF for 30 min (right panels) or kept in serum-deprived media (left panels). Photographs are double exposures with EGFR localization in the green channel (arrows) and Rab5 localization in the red channel (arrowheads). Size bar = 20 µm.
