. Author manuscript; available in PMC: 2024 Aug 1.

Published in final edited form as: Mol Microbiol. 2023 Jun 30;120(2):224–240. doi: 10.1111/mmi.15114

TABLE 1.

Promoter activities of selected genes involved in D-glucose degradation via the spED pathway in Haloferax volcanii.

		β-galactosidase activity (mU/mg)		Induction (fold)
Promotor of	Strain	CAS CAS+D-glucose		Induction (fold)
gad (HVO_1488)	wt	48 ±18	471 ± 84	9.8
	ΔgfcR	47 ±22	58 ± 30	1.2
gapl (HVO_0481)	wt	218 ± 21	585±191	2.7
	ΔgfcR	220 ± 44	221 ± 60	1.0
pykA (HVO_0806)	wt	65 ± 24	141 ± 26	2.2
	ΔgfcR	58 ± 15	72 ± 8	1.2
pgml (HVO_2516)	wt	28 ± 10	26 ± 4	0.9
	ΔgfcR	17 ± 1	13 ± 3	0.8

Note: Promotor activities were measured in a β-galactosidase assay in wild-type and ΔgfcR cells grown on casamino acids (CAS, 0.2%) and after induction by 5 mM D-glucose (CAS+D-glucose). All experiments were carried out in triplicates.