. Author manuscript; available in PMC: 2024 Aug 1.

Published in final edited form as: Mol Microbiol. 2023 Jun 30;120(2):224–240. doi: 10.1111/mmi.15114

TABLE 2.

Promoter activities of selected genes involved in D-fructose degradation via modified EM pathway in Haloferax volcanii.

		β-galactosidase activity (mU/mg)
Promotor of	Strain	CAS	CAS+D-fructose	Induction (fold)
ptfC (HVO_1499)	wt	43 ± 19	214 ± 98	5.0
	ΔgfcR	39 ± 10	44 ± 7	1.1
fba (HVO_1494)	wt	22.0 ± 1^a	52 ± 7^a	2.4
	ΔgfcR	7.0 ± 0^a	8 ± 2^a	1.1
gapl (HVO_0481)	wt	218 ± 21	736 ± 159	3.4
	ΔgfcR	220 ± 44	185 ± 55	0.8
pykA (HV0_0806)	wt	65 ± 24	91^b	1.4
	ΔgfcR	58 ± 15	41^b	0.7

Note: Promotor activities were measured in a β-galactosidase assay in wild-type and ΔgfcR cells grown on casamino acids (CAS, 0.2%) and after induction by 5 mM D-fructose (CAS+D-fructose).

Basal promotor activity of fba was measured in cells grown on 5 mM D-glucose rather than on casamino acids. Experiments were carried out in triplicates.

Single measurements.