. Author manuscript; available in PMC: 2024 Aug 1.

Published in final edited form as: Mol Microbiol. 2023 Jun 30;120(2):224–240. doi: 10.1111/mmi.15114

TABLE 4.

Promoter activities of selected genes involved in D-galactose and glycerol degradation in Haloferax volcanii.

		β-galactosidase activity (mU/mg)			Induction (fold)
Promotor of	Strain	CAS	CAS+D-galactose	CAS+glycerol	D-galactose	Glycerol
HV0_A0330	wt	76 ± 14	260 ± 54	n.d.	3.4
	ΔgfcR	78 ± 18	287 ± 38	n.d.	3.7
glpK (HVO_1541)	wt	106^a	n.d.	202^a		1.9
	ΔgfcR	121^a	n.d.	180^a		1.5
gapl (HV0_0481)	wt	218 ± 21	822 ± 128	344 ± 48	3.8	1.6
	ΔgfcR	220 ± 44	263 ± 37	147 ± 29	1.2	0.7
pykA (HV0_0806)	wt	65 ± 24	102^a	n.d.	1.6
	ΔgfcR	58 ± 15	35^a	n.d.	0.6

Note: Promotor activities were measured in a β-galactosidase assay in wild-type and ΔgfcR cells grown on casamino acids (CAS, 0.2%) and after induction by 5 mM D-galactose (CAS+D-galactose) or 5 mM glycerol (CAS+glycerol). Experiments were carried out in triplicates. Abbreviation: n.d., not determined.

Single measurements.