. Author manuscript; available in PMC: 2024 Aug 1.

Published in final edited form as: Mol Microbiol. 2023 Jun 30;120(2):224–240. doi: 10.1111/mmi.15114

TABLE 5.

Identification of inducer molecules of GfcR by differential scanning fluorimetry (DSF).

Effector	ΔTm (°C)
No effector	0 ± 0.4
D-Glucose	−0.8 ± 1.4
D-Gluconate	−0.01 ± 0.8
2-Keto-3-deoxygluconate	−1.0 ± 1.7
2-Keto-3-deoxy-6-phosphogluconate	+3.1 ± 0.7
D-Fructose	−0.2 ± 0.3
Fructose-1-phosphate	+0.06^a
Fructose-1,6-bisphosphate	+1.5 ± 0.6
D-Galactose	+0.4 ± 0.26
2-Keto-3-deoxy-6-phosphogalactonate	+3.7^a
Glycerol	+0.01 ± 0.02
Glycerol-3-phosphate	+2.8 ± 0.25
Glyceraldehyde-3-phosphate	−0.4^a
DHAP	+0.3 ± 0.06
3-Phosphoglycerate	−0.15 ± 0.07
PEP	−0.7^a
Pyruvate	−0.3 ± 0.2

Note: GfcR (1–5 μM) and 5 mM of ligand were used. Melting temperature (T_m value) of GfcR without ligand corresponds to 70.0 ± 0.4°C. ΔT_m values indicate a shift of melting temperature of GfcR in the presence of ligand; values are given with standard deviations (SD).

Single measurements.