HepG2, HEK293 and MCF7 cells were incubated at 50,000 cells/well for 24 hours individually in comparison to PHHs (50,000 cells/well) incubated with 1.0 μM BPS at 0, 15, 30, 60, 90, 150 and 240 minutes. LC-MS analysis was performed to monitor the clearance of BPS parent compound at 1.0 μM to derive apparent half-life (t1/2). BPS clearance in PHHs (A); BPS clearance in HepG2 cells (B); BPS clearance in HEK293 cells (C); BPS clearance in MCF7 cells (D).