Table 2.
Metabolism Comparison between PHHs and Tox21 Cell Lines for Hydroxybupropion Formation (pmol/min-106 cells); CYP2B6
| Bupropion (μM) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Cell Lines | Incubation Time | 0.1 | 1 | 10 | 62.5 | 100 | 125 | 250 | 500 | 1000 |
|
| ||||||||||
| PHH | 30 min | - | - | - | 33.1 ± 5.14 | - | 38.7 ± 1.34 | 34.5 ± 3.70 | 46.9 ± 3.10 | 64.4 ± 2.60 |
| HepG2 | 24 h | 0.00 | 0.00 | 0.0146 ± 0.000928 | - | 0.00305 ± 0.000668 | - | - | 0.0141 ± 0.000381 | 0.0262 ± 0.00206 |
| HEK293 | 24 h | 0.00 | 0.00 | - | 0.00 | - | - | 0.00203 ± 0.000380 | 0.00262 ± 0.000174 | |
| Hela | 24 h | 0.00 | 0.00 | - | 0.00 | - | - | 0.00245 ± 0.00218 | 0.00447 ± 0.000947 | |
| MCF7 | 24 h | 0.00 | 0.00 | - | 0.00 | - | - | 0.0119 ± 0.0206 | 0.0382 ± 0.00250 | |
| HCT116 | 24 h | 0.00 | 0.00 | - | 0.00 | - | - | 0.00667 ± 0.000451 | 0.00958 ± 0.000485 | |
| C3H10T1/2 | 24 h | 0.00 | 0.00 | - | 0.00 | - | - | 0.0257 ± 0.00337 | 0.0241 ± 0.000575 | |
| ME180 | 24 h | 0.00 | 0.00 | - | 0.00 | - | - | 0.00 | 0.00 | |
| CHO-K1 | 24 h | 0.00 | 0.00 | - | 0.00 | - | - | 0.00 | 0.00 | |
| GH3TRE-Luc | 24 h | 0.00 | 0.00 | - | 0.00 | - | - | 0.00 | 0.00 | |