Table 6.
Chemicals Clearance Comparison between PHHs and Tox21 Cell Lines
| Chemicals | Concentration (μM) | Cell Culture Model | Percent Depletion 240 min (% ±/- SD) | Percent Depletion 24 hour (% ±/- SD) | Mean Half-life (min)* | 95% Confidence Interval Half-life (min) | Fit Method Description |
|---|---|---|---|---|---|---|---|
| 7-HC | 1 | 50-donor pool PHHs | 99 ±/- 5.9 | Not determined | 22.7 | 19.8 – 26.1 | †One-phase exponential decay |
| 7-HC | 1 | HepG2 | 27 ±/−6.8 | 76 ±/- 11 | 290.6 | 196 – 512 | One-phase exponential decay |
| 7-HC | 1 | HEK293 | 50 ±/- 12 | †99 ±/- 12 | 209.9 | 173 – 259 | †One-phase exponential decay |
| 7-HC | 1 | MCF7 | 40 ±/- 18 | 99 ±/- 12 | 292.5 | 196 – 530 | †One-phase exponential decay |
| BPAF | 1 | 50-donor pool PHHs | 82 ±/- 2.9 | Not determined | 86.3 | 51.2 – 202 | One-phase exponential decay |
| BPAF | 1 | HepG2 | 36 ±/- 8.2 | 66 ±/- 6.6 | 31.2 (fast) 1,626 (slow) | very wide | Two-phase exponential decay |
| BPAF | 1 | HEK293 | 36 ±/- 5.4 | 60 ±/- 4.5 | 43.0 (fast) ~1,887 (slow) | very wide | Two-phase exponential decay |
| BPAF | 1 | MCF7 | 42 ±/- 7.7 | 55 ±/- 7.4 | 23.3 (fast) ~9,684 (slow) | very wide | Two-phase exponential decay |
| BPS | 1 | 50-donor pool PHHs | 59 ±/- 6.7 | Not determined | 205.2 | 73.5 – 268 | One-phase exponential decay |
| BPS | 1 | HepG2 | 17 ±/- 11 | 39 ±/- 7.4 | 326.1 | 164 – 3053 | One-phase exponential decay |
| BPS | 1 | HEK293 | 9.5 ±/- 11 | 36 ±/- 12 | NC | NC | One-phase exponential decay |
| BPS | 1 | MCF7 | 22 ±/- 7.0 | 26 ±/- 7.3 | 119.7 | 48.9 – 272 | One-phase exponential decay |
*
value from 150 minutes
†
value applies lower limit of quantification (LLOQ) where mean concentrations less than LLOQ
NC: Not calculated due to insufficient trend within dataset for fit model estimates