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Fig. 2. Effects of cell cycle stage on MCM3 acetylation. The soluble protein fraction and the structure-bound protein fraction prepared from 5 × 105 cells (for anti-acetyl Lys antibody) or from 1 × 105 cells (for other antibodies) treated with nocodazole or mimosine were analyzed by 7.5% SDS–PAGE. The proteins were blotted to PVDF membrane. The membranes were incubated with the indicated antibody, respectively.
