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. 2024 Feb 3;15:1027. doi: 10.1038/s41467-024-45385-x

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Schematic overview of the experimental design used to perform chromosome enumeration using Tigerfish probe sets in 46, XY interphase nuclei also labeled with probe sets targeting non-repetitive DNA designed by PaintSHOP. b Representative images of nuclei labeled with Tigerfish probe sets (magenta) and PaintSHOP probe sets (yellow) targeting intervals on chr22 (top row) or chrX (bottom row). c Heatmap displaying the observed distribution of Tigerfish and PaintSHOP puncta per nucleus. The data presented are aggregated over all the 24 chromosomes targeted. Nuclei with >2 puncta most likely contain replicated sister chromatids that have separated. Images are maximum intensity projections in Z. Scale bars, 10 μm. Each staining pattern was visualized in three independent samples and yielded similar results.