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. 2024 Feb 3;14:2856. doi: 10.1038/s41598-024-52992-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Western blot analysis of total HSP27 expression in SKOV-3 and SKOV-3.tr cell lines with J2 inhibition. (A) Images of Western Blot analysis of total HSP27 in SKOV-3 cells subjected to 10 μM J2 treatment and strain stimulation (30 min). (B) Images of Western blot analysis of total HSP27 in SKOV-3.tr cells subjected to 10 μM J2 treatment and strain stimulation (30 min). Cropped images shown; full membranes in Supplemental Data section. All controls (CTL) were plated on Flexcell plates but were not exposed to strain. (C) Quantification of normalized HSP27 expression Western blot samples; Legend: Blue represents SKOV-3 samples and red represents SKOV-3.tr samples. All bands were normalized to β-actin as a loading control. Data shown as average + SEM; N = 3 samples; *p < 0.05. Samples were compared with ANOVA and post-hoc Tukey HSD tests.