Figure 8.

HSP27 expression in 3D TME models pre-treated with J2 and treated with paclitaxel. (A–D) Representative fluorescence images of microtissue regions in devices with SKOV-3 cells treated with 10 µM of J2 before 24 h of strain compared to control (non-strained) cells. (E) Quantification of HSP27 expression normalized to DAPI fluorescence. Data shown as average + SEM; N = 3, *p < 0.1; scale bar = 200 µm. Solid color bars are SKOV-3 samples; lines represent SKOV-3.tr samples. Samples were compared with Kruskal–Wallis tests followed by post-hoc Dunn’s tests.