Fig. 3. (A) Autoradiograph of the Rad53p in situ autophosphorylation assays. Wild-type or yku80 mutant strains were grown in the presence of 0.02% MMS at 30°C for 2 h, or at 25, 30 or 37°C for 5 h. (B) yku80 mutant strains containing YEP13 (2µ), YEP13–EST2, –TLC1 or –YKU80 were grown at 25, 30 or 37°C for 5 h. (C) Strains were grown at 25 or 37°C, or with 0.005% MMS (M) or 20 µg/ml phleomycin (P), and Rad53p autophosphorylation was assayed as above.