Fig. 5. (A) Southern blot analysis of telomeres from various yeast strains. (B) Top, Rad53p in situ autophosphorylation of strains grown at 25 or 37°C. Bottom, Western blot analysis of Rad53p was performed on 12% polyacrylamide gels. (C) Strains were spotted in 10-fold serial dilutions onto YPAD plates and grown at 30, 36 or 37°C.