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DNA Amplification and PCR protocol for gene polymorphism detection.
| Amplification condition | ||
|---|---|---|
| Components | Volume (µl) | |
| Nuclease free water | 5 | |
| Taq master | 10 | |
| Forward primer | Outer | 1 |
| Inner | 1 | |
| Reverse primer | Outer | 1 |
| Inner | 1 | |
| DNA template | 1 | |
| Total reaction volume | 20 µl | |
