Table 3.

PCR program used for the amplification.

PCR program
PCR cycles		Temperature	Time
Initial denaturation		95 °C	5 min
PCR cycling (35 cycles)	Denaturation	95 °C	30 sec.
	Annealing	65 °C	30 sec.
	Extension	72 °C	1 min.
Final extension		72°C	10 min.
Hold		20°C	∞