FIG. 8.

Association of actin with intracytosolic wild-type L. monocytogenes (A, 2 h; B, 5 h) or with the dal dat double mutant (C, 2 h with d-alanine [100 μg/ml] present from 0 to 30 min; D, 5 h with d-alanine present from 0 to 30 min; E, 5 h with d-alanine present continuously) following infection of J774 cells. Photomicrographs in the top row show the binding of FITC-labeled antilisterial antibodies to total bacteria; those below show the binding of TRITC-labeled phalloidin to actin. Arrowheads indicate some actin-associated bacteria in the sparsely infected cells.