FIG. 2.

Influence of dietary protein malnutrition on the interactions between lymphocytes and macrophages. Peritoneal macrophages from normally nourished donors were infected with live, dispersed virulent M. tuberculosis H37Rv at 37°C for 2 to 4 h and then cocultured for 7 days with whole syngeneic splenocytes or splenocytes depleted of CD4 T cells, CD8 T cells, or both. The splenocytes were derived from inbred strain 2 guinea pigs vaccinated with M. tuberculosis H37Ra and maintained on the LP or HP diet. The cultures were harvested and streaked onto M7H10 agar plates. After 3 weeks of incubation, the CFU were counted. The means ± standard errors of the means for four animals in each group are shown.