Fig. 1. Oligosaccharide-dependent degradation of the mutant proteins CPY* and Pdr5*. (A) Isogenic cells were grown to mid-log phase. Equal cell numbers were then incubated with 100 µg/ml cycloheximide and aliquots removed at given time points. Crude protein extract was separated by SDS–PAGE and analyzed by immunoblotting using anti-CPY (CPY*) and anti-HA (Pdr5*) antisera. (B) Protein amounts from at least two independent experiments were quantified and plotted against time, setting the quantity of time point 0 (steady-state level) to 100%.