Table II. Primers for gene deletions.
| Deletion | Name | Sequence 5′ → 3′a |
|---|---|---|
| HTM1 | HR204forKan | tcgatcatgtagcatgcgaagacgatgcgtactcattcacttctatcgatgaattcgagctc |
| HR204revKan | gccattggaagtgagcacaggactatgtttcttgatttgtacaccgtacgctgcaggtcgac | |
| HR204–90u | gcaataaagaaggacgcg | |
| HR204+350L | ccggtcttccagtatagc | |
| UBC6 | UBC6 forKan | gatggtggaaaaccctccaccatatattcttgctcgccccaacgaagattcgatgaattcgagctc |
| UBC6revKan | tgaagaactatcattaggttctttgccatctttcttggaattattttctgcgtacgctgcaggtcgac | |
| UBC6–180u | actaccatcgcatatcgc | |
| UBC6+130L | gttatcattctgatagccg | |
| UBC7 | UBC7forKan | ctcctcaaggagcttcaacagttaattaaagattctccacctggtatatcgatgaattcgagctc |
| UBC7revKan | ctcactcaacatgctcataacacttaatagaattttttctacactttgcgtacgctgcaggtcgac | |
| UBC7–70u | ggcgtttagcgtacgaag | |
| UBC7+130L | agcgtatggcgtatctgg | |
| All | KanMXu | gtattgatgttggacgag |
aBold letters represent sequences specific to plasmid pFA6aKanMX4 for the amplification of the KanMX resistance gene (Wach et al., 1994).