Fig. 2.

circFCHO2 promotes the proliferation, migration, and invasion of melanoma cells in vitro. A, B qRT-PCR analysis of circFCHO2 and FCHO2 mRNA expression in A375 and MV3 cells transfected with circFCHO2 siRNAs or circFCHO2 overexpression vectors. C, D Colony formation assay was used to detect the proliferation ability of melanoma cells with different treatments. E, F CCK-8 assay was performed to detect the proliferation of melanoma cells. G, H Transwell invasion assay was used to detect the invasion ability of melanoma cells following different treatments. I, J Wound healing migration assay was performed to detect the migration ability of melanoma cells with different treatments. The migration of A375 and MV3 cells was photographed at 0 h, 24 h, and 48 h. K Effect of circFCHO2 on apoptosis as determined by Annexin V-FITC staining and FCM detection. Unpaired Student’s t-test, Mann–Whitney U test, Kruskal–Wallis test and one-way ANOVA test were used for the statistical analyses. *P < 0.05; **P < 0.01; ***P < 0.001