Fig. 5.

DND1 inhibits progression of melanoma by mediating the PI3K/AKT signaling pathway. A Based on the expression of DND1, the overall survival curve was performed using Kaplan–Meier methods and analyzed by the log-rank test. Gene expression and survival data of melanoma cohorts were obtained from The Cancer Genome Atlas (TCGA)-SKCM(data format: TPM. Workflow: STAR) DataSet (https://www.cancer.gov/ccg/research/genome-sequencing/tcga). B, C Colony formation assay was used to detect the proliferation ability of melanoma cells with different treatments. D, E CCK-8 assay was performed to detect the proliferation of melanoma cells. F, G Transwell invasion assay was used to detect the invasion ability of melanoma cells following different treatments. H, I Wound healing migration assay was performed to detect the migration ability of melanoma cells with different treatments. The migration of A375 and MV3 cells were photographed at 0 h, 24 h, and 48 h. Unpaired Student’s t-test, Mann–Whitney U test, Kruskal–Wallis test, and one-way ANOVA test were used for the statistical analyses. *P < 0.05; **P < 0.01; ***P < 0.001