Fig. 6.

Elevated DND1 turnovers the circFCHO2-induced malignant phenotype of melanoma. A Colony formation assay was used to detect the proliferation ability of melanoma cells with different treatments. B CCK-8 assay was performed to detect the proliferation of melanoma cells. C Transwell invasion assay was used to detect the invasion ability of melanoma cells following different treatments. D Wound healing migration assay was performed to detect the migration ability of melanoma cells with different treatments. The migration of A375 and MV3 cells were photographed at 0 h, 24 h, and 48 h. E. Western blot assay was used to detect the p-AKT and AKT levels in melanoma cells with different treatment, GAPDH was used as a negative control. Paired Student’s t-test, unpaired Student’s t-test, Mann–Whitney U test, one-way ANOVA test, and Kruskal–Wallis test were used for the statistical analyses.*P < 0.05; **P < 0.01