Fig. 3. Dose-dependent stimulation of the ATPase activity of TouR by o-cresol, m-cresol and p-cresol. TouR (500 nM) was mixed with ATP (0.02 µCi/µl [γ-³²P]ATP, 3 mM ATP) and UAS DNA (250 nM) in the presence of increasing concentrations of each of the cresols. After incubation for 30 min at 30°C, samples were spotted onto TLC plates as in Figure 2 to monitor ATP hydrolysis. Activities are expressed as the percentage of the hydrolysis of ATP achieved by TouR with 100 µM o-cresol added.