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. 2024 Jan 22;15:1294357. doi: 10.3389/fimmu.2024.1294357

Figure 1.

Figure 1

Whole-cell patch clamp experiments were performed with HEK293 cells stably expressing human TRPM2. The intracellular solutions contained 0, 10, 100, 300, 1000 or 10000 nM Ca2+ and 100 µM ADPR/2dADPR. The extracellular solution contained 1 mM Ca2+. Concentration-response curves yielded EC50 values of 694 ± 115 nM (best-fit value ± SE) for ADPR and 192 ± 62 nM for 2dADPR that differed significantly (p<0.01, extra sum-of-squares F test). Currents at [Ca2+]i = 0, 10, 100, 300, 1000 and 10000 nM reached 0.17 ± 0.04 (n=12), 0.18 ± 0.06 (n=9), 0.35 ± 0.06 (n=18), 0.73 ± 0.01 (n=22), 14.6 ± 1.50 (n=8) and 17.6 ± 2.68 (n=10) nA for ADPR and 3.58 ± 0.64 (n=10), 2.89 ± 1.04 (n=6), 4.84 ± 0.67 (n=18), 16.5 ± 3.06 (n=16), 20.9 ± 3.24 (n=8) and 18.2 ± 3.23 (n=12) nA for 2dADPR, respectively. Currents are given as means ± SEM with n determinations. ns not significant, *p < 0.05, ****p < 0.0001 (multiple t test).