Figure 4.

Whole-cell patch clamp experiments were performed 24 h after HEK293 cells were transiently transfected with an expression vector containing mutated (W1355A) or wild type (wt) human TRPM2. The intracellular solution contained 300 nM Ca²⁺ and 100 µM ADPR/2dADPR. For negative controls no agonist was added (buffer). The extracellular solution contained 1 mM Ca²⁺. (A) Resulting maximum whole-cell currents. (B) Time course of normalized whole-cell current over 445 s. Currents are given as means ± SEM. Rates of activation (C) and inactivation (D) were determined as described in Figure 2B . Data in (A, C, D) displayed log-normal distribution and were log-transformed to obtain normal distribution. They are presented on an anti-log scale. The bar denotes the mean. ns not significant, **p < 0.01, ***p < 0.001, ****p < 0.0001 (one-way ANOVA, t-test).