Fig. 2. Assembly of FtsQ is dependent on SecA, SecE and YidC. (A) Proteinase K accessibility of FtsQ (top) and OmpA/bandX (bottom) in BA13 (SecA depletion strain) and DO251 (control strain) spheroplasts, made from cells cultured at 41°C. Cells were labeled and processed as described in Methods. OmpA and band X are periplasmic and cytoplasmic control proteins, respectively. (B) Proteinase K accessibility in CM124 (SecE depletion strain) spheroplasts not depleted and depleted for SecE. (C) Proteinase K accessibility in JS7131 (YidC depletion strain) spheroplasts not depleted and depleted for YidC.