Figure 3.

Effect of Totum-070 on liver lipid metabolism. (A) Frozen mouse liver tissue sections stained with Oil Red O and counterstained with hematoxylin (upper panels). Scale bars, 50 µm. Paraffin-embedded sections of mouse livers stained with hematoxylin and eosin (H&E) (lower panels). Scale bar, 100 µm. (B) Quantification of Oil Red O in liver sections (at least three images per liver, n = 14 per group) shows reduction of neutral lipid accumulation in mice supplemented with Totum-070. (C) Quantification of hepatic triglyceride content (n = 14 per group). (D) Relative expression in liver of selected genes encoding proteins implicated in cholesterol metabolism (n = 14 per group). (E) Immunoblot to monitor LDL receptor (LDL-R) expression in liver samples from the indicated groups and quantification. (F) Relative expression in mouse liver samples from the indicated groups of selected genes encoding proteins implicated in lipid metabolism. (G) Relative expression in mouse liver samples from the indicated groups of selected genes encoding proteins implicated in lipoprotein metabolism. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 vs. ND. #p < 0.05, ##p < 0.01, ###p < 0.001 and ####p < 0.001 Totum-070 (T070) groups vs. WD. Data are the mean ± SEM.