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SDS-PAGE analysis of the OMPs of B. avium 4169 and the transposon mutants Pho-6 and Pho-20. Lanes: 1, molecular mass standards; 2, 4169 in BHI; 3, 4169 in BHI-EDDA; 4, Pho-6 in BHI; 5, Pho-6 in BHI-EDDA; 6, Pho-20 in BHI; 7, Pho-20 in BHI-EDDA. Lanes 2 to 7 each contain 1 μg of total protein. Molecular masses are in kilodaltons. The positions of the 95- and 84-kDa FeRPs that are missing in the outer membrane of Pho-20 are denoted by arrows. The gel was stained with silver.
