TABLE 1.

Formula for the CDM for growth of B. avium^a

Step	Ingredient or procedure	Amt
Base solution	KH2PO4	0.5 g
	Sodium glutamate	10.72 g
	NaCl	2.5 g
	KCl	0.2 g
	MgCl2 · 6H2O	0.1 g
	Tris	1.525 g
	α-Ketoglutamic acid	2.0 g
	Sodium pyruvate	2.0 g
	CaCl2	20.0 mg
	Deionized H2O	To 909.0 ml
	Adjust to pH 7.8 with NaOH, autoclave, and store at 4°C
CDM supplement	Ascorbic acid	0.2 g
	Glutathione, reduced	1.0 g
	Deionized H2O	To 100.0 ml
	Store at −20°C
l-Cystine supplement	Deionized H2O	3.0 ml
	HCl (11.6 M)	1.0 ml
	l-Cystine	0.4 g
	Deionized H2O	To 100.0 ml
	Store at −20°C
l-Proline supplement	l-Proline	3.0 g
	Deionized H2O	To 200.0 ml
	Autoclave and store at −20°C
l-Phenylalanine supplement	l-Phenylalanine	4.0 g
	Deionized H2O	To 400.0 ml
	Autoclave and store at 4°C
Vitamins	p-Aminobenzoic acid	10 mg
	Biotin	1 mg
	Folic acid	10 mg
	Pyridoxyl HCl	80 mg
	Riboflavin	40 mg
	Thiamine HCl	40 mg
	Calcium pantothenate	80 mg
	Nicotinamide	200 mg
	Deionized H2O	800 ml
	Add 2.5 N NaOH until solution clears
	Add deionized H2O to a final volume of 1,000 ml
	Store at −20°C
Prepare final medium	Base medium	909 ml
	l-Cystine stock	10 ml
	l-Proline stock	16 ml
	CDM supplement	10 ml
	l-Phenylalanine stock	40 ml
	Vitamins	10 ml
	Sterilize by ultrafiltration (0.2-μm pore size)

^aCDM may be stored for up to 2 weeks at 4°C. Stock solutions may be stored for up to 3 months.