TABLE 2.
Guinea pig DTH responses to purified proteins of M. tuberculosis
| Antigen | Diam (mm) of skin test reactions in guinea pigs immunized witha:
|
SpIb | |
|---|---|---|---|
| M. bovis BCG | M. avium | ||
| Tuberculins | |||
| PPD-t | 8.9 ± 1.3 | 8.0 ± 1.4 | 1.11 |
| PPD-b | 12.1 ± 1.3 | 9.5 ± 1.5 | 1.27 |
| PPD-a | 8.0 ± 1.5 | 12.6 ± 1.4 | 0.63 |
| Cross-reactive antigens | |||
| 19 kDa | 7.0 ± 2.0 | 9.3 ± 1.6 | 0.75 |
| MPT51 | 6.2 ± 0.5 | 5.0 ± 0.7 | 1.24 |
| Ag85B | 7.9 ± 2.1 | 8.9 ± 0.8 | 0.89 |
| 38 kDa | 6.4 ± 0.9 | 5.0 ± 0.8 | 1.28 |
| MPT32 | 7.2 ± 1.3 | 7.8 ± 1.7 | 0.92 |
| KatG | 7.4 ± 1.2 | 4.1 ± 0.9 | 1.80 |
| Specific antigens | |||
| MPT63 | 7.5 ± 1.6 | 0.5 ± 1.0 | 15.00 |
| MPT70 | 7.6 ± 1.2 | 1.0 ± 1.2 | 7.60 |
| MPT64 | 7.6 ± 1.0 | 0.5 ± 1.0 | 15.20 |
| MTC28 | 6.6 ± 1.4 | 0.8 ± 1.5 | 8.25 |
a
Means ± standard deviations of diameters of erythema after intradermal injection of 2 μg of each purified antigen in six guinea pigs immunized with M. bovis BCG and six guinea pigs immunized with M. avium, as described in Materials and Methods; 10-TU doses of PPD from M. tuberculosis (PPD-t), M. bovis (PPD-b), and M. avium (PPD-a) were used as controls for sensitization.
b
Determined by dividing mean diameters of skin reactions measured in BCG-immunized guinea pigs by those in M. avium-immunized animals.