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The elastase inhibitory capacity (EIC) of porcine serum. Human neutrophil elastase (400 ng) in 10 μl of saline (0.15 M NaCl) or saline-serum mixture (serum), or a mixture of elastase with serum exposed to activated porcine neutrophils (conditioned serum), was assayed on an elastin-agar plate. Mixtures of human neutrophil elastase (400 ng) with varying amounts of purified α1-PI were used as standards for the inhibition of elastase activity. Data are means from two experiments.
