FIG. 8.

Bactericidal activity of latex-stimulated neutrophil secretions in a gel-overlay bactericidal assay. Shown are results of two gel-overlay assays with E. coli and L. monocytogenes. Neutrophil secretions were prepared as described for Fig. 5. Ten million cell equivalents of neutrophil secretion was used in each lane of the overlay assays. PG, 0.5 μg of synthetic PG3, used as positive control for the activity and identification of protegrins. Antibacterial activity was indicated by a clear zone (no bacterial growth).