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Fig. 2. TNF resistance in Metamut cells was demonstrated by different assays. Wild-type parental L929 cells (open circle), Metamut cells (filled circle), reconstituted cells (open square), and vector control cells (filled square) were treated with TNF or TNF + cycloheximide (CHX, 0.3 µg/ml) for different periods of time. Cell viability was measured by MTT assay (A), annexin staining (B), or PI staining (C and D).
