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. 2024 Jan 11;27(2):108877. doi: 10.1016/j.isci.2024.108877

Figure 1.

Figure 1

In FP primed/trimer boosted NHPs, long trimer interval, but not long FP prime interval, significantly impacts plasma anti-base responses

For all regimens, NHP plasma at three key time points of two weeks after the fifth FP immunization, the first trimer immunization and the second trimer immunization were analyzed (indicated with a red arrow). Two groups of five animals each were first primed three times with FP8-KLH followed by FP7-KLH, FP6-KLH, and two BG505 DS-SOSIP immunizations, with Adjuplex and I.M. Green and black circles and lines represent NHPs from short interval groups and long-interval groups, respectively.

(A) One group of five animals were immunized with short intervals at weeks 0, 4, 8, 12, 20, 24, and 28, and one group of five animals were immunized with long intervals at weeks 0, 4, 20, 32, 44, 56, and 64.

(B) Plasma binding to BG505 DS-SOSIP by ELISA as measured by OD450nm plotted against plasma dilution factors for short and long interval groups at the three key time points of two weeks post fifth FP (left panel), two weeks post first trimer (second panel from left) and two weeks post second trimer (right panel).

(C) Total trimer response as measured by ELISA BG505 DS-SOSIP AUC from the values in B.

(D) Comparison of the total ELISA OD450nm values for binding to BG505 DS-SOSIP with (hollow circles) and without (solid circles) competition with RM19R Fab between the short and long interval groups at dilutions of 1:20, 1:50, and 1:500 for the three time points, respectively.

(E) Percentage of response targeting the base region calculated from the values in D. The percentage was calculated by taking the difference between OD450 values without and with RM19R Fab, divided by the OD450 values without RM19R Fab blocking, and multiplied by 100.

(F) Plasma binding by ELISA to glycan-base BG505 DS-SOSIP trimer, which has glycans added on the base region.

(G) Trimer response as measured by AUC from the values in F. Two-tailed Mann-Whitney nonparametric tests were used for statistical analysis to assess p values for mean ± SEM. ∗: p < 0.05; ∗∗: p < 0.01; ∗∗∗: p < 0.001; ∗∗∗∗: p < 0.0001; ns, not significant. The data in C represent two independent experiments with similar results.

See also Figure S1.