Fig. 4. Jab1 inhibits TGF-β-mediated transcription. Mv1Lu cells were transfected with (SBE)4-luc (A), p6MBE-luc (B), ARE-luc and Fast-1 expression plasmid (C) and 4¥AP1 MT (D) or together with indicated doses of Jab1 expression vector. TGF-β (2 ng/ml) was added in some experiments. Twenty-four hours after transfection, the cells were lysed and the luciferase signal was detected.