Figure 2.

Embigin deficiency increases mortality from embryonic day E8.5 to P3

(A) The relative frequency of the genotypes of the pups from heterozygous Emb^+/− breedings were determined. Three litters at E8.5; 6 litters at E9.5; 1 litter at E10.5, E11.5, and E14.5; 2 litters at E16.5, and 10 litters at E17.5 were examined (n = a number of pups analyzed). The correlation between the frequency of each genotype and the embryonic stage was analyzed by calculating Spearman’s rank correlation coefficient (r_s) and its statistical significance.

(B) The relative frequency of the genotypes of the pups from Emb^+/− breedings was determined. 284 pups from 40 litters were analyzed at P14-P21.

(C) To determine the postnatal survival frequency of the Emb^−/− mice, 100 pups from 17 litters and 6 different Emb^+/− breedings were followed after birth. The genotypes were determined after the death of the pup.

See also Figure S2 and Table S1.