Fig. 10.

Lipidomic analysis of hepatocytes treated with adipocyte conditioned media. A: The workflow highlighting the production of conditioned media and the treatment of hepatocytes. B: Pie diagram highlights targeted LC-MS analysis of hepatocyte lipidomic analysis grouped by corresponding lipid class. C: Heatmap displays all lipid molecular species comparing hepatocytes treated with conditioned media from adipocytes treated with vehicle or CL-316,243 (n = 6). D: Stacked bar plot with major lipid species comparing hepatocytes treated with adipocyte conditioned media. E: The major lipid classes were grouped by major molecular species. F: Overlapping of characterized lipids species, three Venn graphics present All lipid species overlapping, CL significantly induced lipids overlapping (P < 0.05), and CL significantly repressed lipids overlapping (P < 0.05). Red pie represents liver lipidome after 5 h CL treatment. Blue pie represents hepatocyte lipidome after condition medium treatment. Aqua pie represents condition medium lipidome. G: Real-time PCR analysis of HEPA 1–6 cell line in response to condition media from differentiated adipocytes. H: Real-time PCR analysis of mouse liver organoids treated with adipocyte conditioned media. In all experiments, conditioned media was collected from adipocyte after 5 h incubation with saline or CL-316,243.