Fig. 4.

(E)-trans-2-nonenal toxicity in a G. mellonella larvae model. (A) Survival curve of G. mellonella larvae infected with different (E)-trans-2-nonenal concentrations. Larvae were monitored up until 48 h post-inoculation. (B) Dose-response curve of different (E)-trans-2-nonenal concentrations for lethal dose determination. Lethal dose (LD₅₀) was determined as 6.896 g (E)-trans-2-nonenal per Kg of larvae. Assuming 0.2 g and 10 μL-volume inoculation per larvae, the (E)-trans-2-nonenal LD₅₀ is 138 mg/mL; R² = 0.9697. Log-rank tests were conducted between Kaplan-Meier curves to evaluate statistically significant differences. Asterisks show differences compared to the control.