Figure 3. Cytokine expression in CD4+ and CD8+ T cells found in blood of macaques 2 months following last immunization.

PBMCs from immunized macaques are stimulated with growth medium, ConA, SIV gag, HIV env or SIV pol peptides for 5 hours and then cells are stained for phenotypic markers and analyzed by flow cytometry. (A) Gating strategy of cytokine expression. (B) Average expression of cytokines IFN-γ, IL-2, and TNF-a in CD4+ /CD8+ T cells.