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Chromatographic conditions in analysis of QJHTD components.
| Parameter | Value |
|---|---|
| Sample injection volume | 5 μL |
| Separating column | Agilent Eclipse XDB C18 column (250 mm × 4.6 mm, 5 μm) |
| Column temperature | 30 °C |
| Mobile phase A | acetonitrile |
| Mobile phase B | 0.1 % phosphoric acid solution |
| Flow rate | 1 mL min−1 |
| Detection wavelength | 230 nm |
| Theoretical plate number | ≥2500 (as calculated upon baicalin peak) |
