Table 3.

Gradient elution for constituent detection of QJHTD extract.

Elution time (min)	A: acetonitrile (%)	B: 0.1 % phosphoric acid solution (%)
0	5	95
5	6	94
25	15	85
35	21	79
37	24	76
48	25	75
53	26	74
56	28	72
65	100	0
76	100	0

Dexamethasone (DEX; Lot No. C84-1434-1G, purity >99 %) as a positive control was purchased from Dalian Meilun Biotech Co., Ltd (Dalian, China).

All agents were thoroughly dispersed in culture medium or deionized water at specific concentrations when used for in vitro or in vivo activity testing.